Testosterone Test
The Testosterone test measures the level of testosterone in your blood. Testosterone is the primary male sex hormone, responsible for sex drive, muscle mass, and fertility. The test is used to evaluate symptoms like low libido, erectile dysfunction, infertility, or irregular periods in both men and women.

The Testosterone test is a blood test that measures the level of testosterone, a vital hormone produced mainly by the testes in men and the ovaries in women, with small amounts also produced by the adrenal glands. Testosterone plays a crucial role in the development and maintenance of male physical characteristics, sexual function, muscle and bone mass, and mood regulation. It is also important for women’s hormonal balance and overall health, though in smaller amounts.
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Why the Testosterone Test is Done:
In Men:
Evaluate erectile dysfunction
Assess low sex drive
Diagnose infertility
Investigate delayed or early puberty
Detect testicular or pituitary disorders
Monitor testosterone replacement therapy
In Women:
Investigate excessive hair growth (hirsutism)
Diagnose polycystic ovary syndrome (PCOS)
Examine irregular menstrual cycles
Check for virilization (development of male traits)
In Children:
Assess abnormal puberty development
Diagnose delayed or precocious puberty
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Types of Testosterone Measured:
Total Testosterone: Includes both free and bound testosterone.
Free Testosterone: The biologically active portion not bound to proteins.
Bioavailable Testosterone: Free plus loosely bound testosterone.
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Sample Required:
A blood sample, preferably taken in the morning when testosterone levels are highest.
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Preparation:
Typically no fasting required.
Should be done in the morning (7–10 a.m.) for accurate results in men.
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Normal Ranges
(can vary by lab):
Group Total Testosterone Level
Men (adult) 300 – 1000 ng/dL
Women (adult) 15 – 70 ng/dL
Children Varies by age and puberty stage
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High Testosterone Levels May Indicate:
Tumors of the testes or adrenal glands
Anabolic steroid use
Congenital adrenal hyperplasia (CAH)
PCOS in women
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Low Testosterone Levels May Indicate:
Hypogonadism (underactive testes)
Pituitary gland disorders
Chronic illness or obesity
Aging (age-related decline)
Stress or alcohol abuse
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Benefits of the Testosterone Test:
Diagnoses male and female hormone disorders
Helps guide treatment for infertility and sexual dysfunction
Supports diagnosis of PCOS and endocrine disorders
Monitors effects of hormone replacement therapy
The Testosterone test is an essential tool for evaluating reproductive health, sexual function, and hormonal balance. It provides valuable insights into conditions related to both androgen excess and deficiency across all genders and age groups.